![]() ![]() In addition, this project should lead to the identification of CPK3 nuclear targets and thus bring some evidences on how a single calcium sensor both involved in abiotic and biotic responses can drive plant response specificity. Expected results should improve our knowledge on a still unresolved signaling pathway involved in plant-pathogen interaction. For this purpose, a comparative phosphoproteomic study will be conducted on nuclear proteins extracted from various plant lines (WT and cpk3 knock-out lines) treated or not with calcium inhibitors and Fumonisin B1. The current project aims at confirming this hypothesis through microscopy approaches and to evaluate the importance of nuclear calcium in the activation of the nuclear form of CPK3. Preliminary data also indicates that CPK3 (full length or cleaved form) migrates into the nucleus. ![]() Upon treatment of Arabidopsis cells with a mycotoxin that modulates the sphingolipid metabolism and induces PCD (Fumonisin B1) it has been shown that CPK3 is cleaved. In the programmed cell death (PCD)-i nduced sphingolipid signaling it has been shown that nuclear calcium plays a crucial role and that a calcium-dependent protein kinase (CPK3) is involved in the pathway. Calcium is now recognized as a crucial second messenger regulating most of signaling pathways involved in plant development and plant defense against pathogens. ![]()
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